Testing of ENDS aerosol collected condensate in the in vitro toxicology assays is limited by OECD guidelines for the maximum allowable solvent levels that can be tested, as well as the final concentration of the generated aerosol condensate. The concentration of condensates collected using organic solvents such as ethanol, are limited in the neutral red uptake (NRU) assay to 0.5 % of vehicle in the dosing medium. Using our standard linear smoking machine collection procedure for ENDS aerosol in ethanol yields an average final condensate concentration of 60 mg/ml, with a resultant maximum NRU testing dose of 300 ug/mL. Testing of higher doses requires exceeding OECD guideline solvent limits or the generation of more concentrated condensates. Here we describe the development and characterization of a proprietary ENDS aerosol collection device designed to smoke multiple devices in sequential order to generate a continuous aerosol stream with similarities to a rotary smoke machine. Puffing is accomplished using a dual puffing engine and the aerosol generated is captured onto a 92 mm pad. This setup allows for collection of larger amounts of aerosol with final concentrations of ~200 mg/ml, thereby tripling the standard collection concentration. An additional advantage with this novel aerosol collection method is that it allows for collection times that are 10 times faster when compared to the standard linear smoking machine collection. Condensates collected from the standard linear smoking method and this novel alternative rotary-like device were characterized for nicotine and carbonyl levels, and response in the NRU assay. The nicotine and carbonyl levels measured were comparable between the two aerosol collection methods. Condensates collected from both collection methods, under non-intense and intense regimes, were compared in the NRU assay using 3T3 cells and A549 cells.