Blue mold caused by Peronospora tabacina is a foliar disease that can be a serious problem in tobacco production. The development of blue mold disease in the field is greatly influenced by environment, which complicates the process of breeding for resistance. In previous work, random amplified polymorphic DNA (RAPD) markers linked to blue mold resistance derived from Ovens 62 were identified. However, RAPD markers can be very sensitive to reaction conditions and take longer to analyze than standard PCR reactions. In order to simplify the characterization of breeding lines, two of the RAPD markers linked in coupling to blue mold resistance were cloned and sequenced. The sequences had no significant similarity to known sequences. These cloned sequences were used to design primers for sequence-characterized amplified region (SCAR) markers for simpler identification of lines containing the markers. These SCAR markers have been used in marker-assisted selection to identify blue mold resistant breeding lines. Breeding lines with and without these markers are being evaluated in field trials in order to determine if blue mold resistance is reliably predicted by the presence of the SCAR markers.