In order to investigate the characteristics of tobacco introduced from Zimbabwe and screen the cultivar best suitable for Yuxi in Yunnan, the density and exudates of glandular trichome in matured upper, middle and lower leaves of 4 introduced cultivars (KRK22, KRK23, KRK26 and T29) and 2 major cultivars (K326 and Honghuadajinyuan) in Yunnan were compared by living body microscopic observation and GC/MS analysis. The results showed that: 1) The glandular trichome density differed significantly among cultivars, that of Honghuadajinyuan and K326 were the largest and significantly higher than that of KRK22 and KRK23. 2) The glandular trichome exudate content differed significantly among varieties, that of Honghuadajinyuan and K326 were the highest and significantly higher than that of KRK23. The density and exudate content of glandular trichome significantly positively correlated, though not linearly. In view of that tobacco quality closely related to the density and exudates of glandular trichome, Honghuadajinyuan and K326 were better than the introduced cultivars, particularly KRK23.