Two experiments investigated the effects of various treatments on TSNAs (tobacco-specific nitrosamines) in Burley tobacco (sealed cut rag) after high temperature storage. In Experiment 1, tobacco with five moisture contents (6%, 12%, 17%, 23%, 31%) was stored at 45 °C for 15 days. From the initial level of 0.5 ppm, TSNAs increased most in the low moisture tobacco; to 5.0 and 4.2 ppm in the 6% and 12% moisture, respectively, and less in the three higher moisture treatments (1.7-1.9 ppm). In Experiment 2, nitrate (1 g / 20 g tobacco), nitrite (0.1 g / 20 g tobacco) and a water check were sprayed separately on low (8%) and high (18%) moisture tobacco before storage at 60 °C for 15 days. From an initial TSNA level of 3.8 ppm, TSNAs in the water check increased after storage to 77.1 and 33.2 ppm in the low and high moisture tobacco, respectively. Nitrite sprays caused a very marked increase in both the low and high moisture tobacco, to 230.0 ppm and 207.4 ppm, respectively. The addition of nitrate caused a much smaller increase in the high moisture tobacco but a marked increase in the low moisture tobacco, to 195.1 ppm, suggesting that nitrate may be more stable in the high moisture tobacco.