We found that the tobacco filling ratio of tobacco columns influences the ratio of nicotine in smoke to tar ( i.e. NT ratio). We reported it at the CORESTA Smoke & Technology Joint Meeting in 2001. At that time we only focused on the variation of NT ratio by the tobacco filling ratio, and the tobacco filling ratio and NT ratio showed a nonlinear sigmoid relation. Since then, we paid attention to the average angle that crosses the cut tobacco to the longitudinal direction in tobacco columns (orientation angle). In our study, the tar and nicotine yields were determined in accordance with ISO 3308 and ISO 4387. The cut tobacco weight and tobacco rod pressure drop were measured in accordance with ISO 6565. The orientation angle was measured non-destructively using compound X-ray inspection system. In the study, we found the NT ratio had increased in proportion almost linearly to the increase of the tobacco filling ratio in each level of the orientation angle. Accordingly, we thought that it was because the filtration ratio of tar and that of nicotine varied by the tobacco filling ratio. On the other hand, the sine of orientation angles showed linear correlation with NT ratio. We assumed that it was because the orientation angle influences NT ratio. We will, therefore, present that the tobacco filling ratio and the orientation angle are factors independent of each other against NT ratio.