Sulfentrazone (Spartan 75 DF) is a relatively new herbicide in flue-cured tobacco production in the US. It provides excellent control of several of the most troublesome weeds in the US and worldwide including Ipomoea sp., and Cyperus sp. Crop injury has been observed with sulfentrazone. Injury tends to be greater when this herbicide is applied pre-plant incorporated, and may be related to concentrated areas of the herbicide in the root zone that are the result of shallow incorporation followed by bedding of tobacco soils before transplanting. Research was conducted in the greenhouse in 1999 to evaluate injury from the placement of sulfentrazone in several zones relative to the root ball of greenhouse produced transplants. Zones of incorporated herbicide were established with a field soil in the top eight cm, bottom eight cm, and throughout a 16 cm deep pot. Zones were separated using activated carbon to prevent movement of herbicide to untreated soil. Growth and phytotoxicity data were then collected.