The objective of this International Nematode Screening program organised by the CORESTA Working Group on Nematodes was to identify sources of genetic resistance to the most important root knot nematode species and races causing damage to tobacco for use in developing varieties resistant to these pests. Fourteen tobacco varieties, eight from CORESTA and six from ARET were screened against nematodes at Kandiya Research Station sick plot in a randomised complete block design with five replications. Seedlings were raised according to recommendation for tobacco production at Kandiya main nursery and were transplanted with the first rains. The plot sizes consisted of four ridges with plants spaced at 0.6 metre within the ridge and 1.2 metres between the ridges. No nematicides or soil fumigants were applied at the test site. However, registered insecticides were used to control insect pests in the nursery. Soil samples of 500 gram each were collected from each plot at planting, 6, 9 and 12 weeks after planting for nematode count and plants were assessed for root gall at 6, 9 and 12 weeks after transplanting on a scale 0 to 10 per plant where 0 meant clean roots and 10 meant that all roots were destroyed with root knot nematodes. The test varieties differed in their reaction to nematodes. Out of 14 entries, three of them, NOD 8, M208 and STNCB were highly resistant to nematode infection. Out of the six Malawi Varieties, none showed some resistance to nematode infection. Varieties B84-1052 and MTRA92 grew tall despite being infected.