The chemical properties of tobacco smoke change very rapidly during the smoking process. A real time analysis is required to better understand the chemistry of smoking. Puff-resolved analysis of tobacco smoke can be done by conventional off-line analysis methods, but can be difficult and time-consuming. For fast on-line, real-time measurements of the tobacco smoking process, time-of-flight mass spectrometry (TOFMS) is the optimal method, as only TOFMS can record simultaneously a range of masses at the required very high measurement rates and precision. The theoretical background of the functionality is described in Part 1: "Scientific background and basic principles". Based on the theoretical background of the functionality measurement method given in Part 1, this presentation gives a review of the components used in the collection of measurements. It also describes the potential issues of coupling an analyser directly in-line with the mainstream smoke path as well as possible solutions. Such are specific solution, measurement point and hysteresis effects. But also the influence of the specific solutions to the measurement results are investigated and discussed.In conclusion a practical configuration will be presented to provide in-line measurement of mainstream smoke.First results with this instrument and expectations for the future will be presented in Part 3 of this presentation: "First measurements with a Smoke Analyser Prototype system".