Nitrogen rates of 168, 224, 280, 336, 392 and 448 kg/ha were evaluated for influence on soil pH, plant vigor, yield, NNN, total TSNA, nitrite and nitrate in burley tobacco at four locations, Fayette, Jackson, Madison and Shelby Counties in Kentucky. Ammonium nitrate (AN) at 840 kg/ha was compared using the same parameters at the same locations with a combination of AN at 504 kg/ha plus 800 kg/ha of 14-0-14, a calcium nitrate/potassium nitrate mix. In Lincoln County 250 kg/ha of nitrogen was applied as 623 kg/ha of urea broadcast, 840 kg/ha AN broadcast, 373 kg/ha urea broadcast plus 336 AN sidedressed, 504 kg/ha AN broadcast plus either 336 kg/ha AN, 772 kg/ha calcium nitrate, 700 kg/ha sodium nitrate or 800 kg/ha of 14-0-14 sidedressed. Soil pH trended downward as nitrogen rates increase. Plant vigor was variable. Yields trended upward as nitrogen rate increased in two locations but was stable across the range of nitrogen rates in the other two locations. NNN, total TSNA, nitrite and nitrate increased as nitrogen rates increased but not significantly. Calcium nitrate in Lincoln County produced significantly better yield than either treatment where urea was used, but did not differ from other nitrogen sources. Different nitrogen sources at Lincoln County caused no change in NNN, total TSNA, nitrite or nitrate. Ammonium nitrate at 504 kg/ha broadcast plus 800 kg/ha of 14-0-14 produced no difference in yield but significantly higher NNN, total TSNA and nitrite levels at the Fayette location when compared to 840 kg/ha of AN, but these difference did not occur at the other three locations.