Pesticide residues could be an important index in the health risk assessment of cigarettes. The transfer amount of pesticide residues into smoke depends on the physical and chemical properties of pesticides. In order to investigate the distribution rules and transfer rates of pesticide residues in cigarettes during smoking, 126 pesticide standards were added to cigarettes at different concentrations, and the transfer rates of these pesticides into mainstream smoke (MSS), sidestream smoke (SSS), ash and butt were determined by sidestream smoking machines under the ISO standard smoking with liquid chromatography-tandem mass spectrometry (LC-MS/MS), gas chromatography-mass spectrometry (GC-MS) and chromatography (GC-ECD) methods. The results showed that: 1) The transfer rates of suckercides into MSS, SSS and butt were 13.16%, 21.74% and 10.27%, respectively; those of organochlorine pesticides, herbicides and pyrethroids were 8.68%-10.11%, 15.72%-17.28% and 5.65%-6.74%, respectively; and those of organophosphorus insecticides, fungicides, heterocyclic insecticides and carbamate insecticides were 1.70%-5.26%, 1.90%-6.11% and 1.22%-4.55%, respectively; 2) With the total transfer rate of 22.96%, the transfer rate of these pesticides into sidestream smoke was the highest, followed by that into mainstream smoke, that into butt was the lowest, while none of these pesticide residues was detected in ash.