A new Burley tobacco KB 101 was developed by single plant selection in the F2 generation derived from double cross, [(Burley 21 x Burley 37)F1 x (Burley 64 x Ky16)F1], and was released in 1987. Burley 64 and Burley 37 were the source of resistance to bacterial wilt (Pseudomonas solanacearum) and black shank (Phytophthora nicotianae) respectively. Yield tests were conducted in the F5 through F6 generations at the four experiment stations of Korea Ginseng & Tobacco Research Institute. On-farm trials were carried out by cooperation with Korea Tobacco & Ginseng Corporation in the F7 through F8 generations at the 45 locations during the three crop years from 1984. KB 101 has resistance to both bacterial wilt and black shank, and has an erect growth habit similar to that of Burley 21, the main Burley cultivar in Korea since the 1960's. Its plant size is larger and it has more leaves than Burley 21. It is a medium-late maturing variety that flowers approximately 3 days later than Burley 21. The average yield of KB 101 was 7% higher than that of Burley 21, and price per kilogram was similar to that of Burley 21. The physical characteristics and chemical composition of KB 101 were similar to those of Burley 21.