Flue-cured tobacco, Nicotiana tabacum L., was grown on a granitic soil (Palestaulf) in Australia for 9 years. Crop rotation treatments for this study were continuous tobacco (1:1), tobacco grown every other year (1:2), and tobacco grown every fourth year (1:4). Soil analyses from treatment plots were compared with those from an adjacent, uncultivated site. After nine years, levels of organic carbon and mineral nitrogen in the 0-30 cm soil profile did not change significantly in any of the crop rotation treatments. In the upper 0-15 cm of the soil compared with the uncultivated site, DTPA-extractable Zn accumulated significantly, Cu and Fe did not change, and Mn declined from 31 to 12 mg/kg. Phosphorous (both acid- and bicarbonate-extractable) accumulated significantly over time in all cropping sequences. Phosphorous rapidly accumulated in the 0-60 cm soil depth and, as the experiment progressed, P moved downward to 60-120 cm. In contrast, K and Ca, which were supplied by the tobacco fertilizer, did not accumulate in the surface profiles. Instead, these cations leached downward to the 45-120 cm depth where they accumulated to 4-5 times that of the uncultivated site. Magnesium, which was only supplied by the irrigation water, accumulated in the 60-120 cm depth to a level twice that of the uncultivated site.