The main varieties in the flue-cured district in Yugoslavia are Podravina and Drava. Both are tolerant to PVY, but rather susceptible to TMV. Over the last three years 1986, 1987, 1988 all genotypes in the comparative tests, at the Zagreb Tobacco Institute experimental station in Pitomaca, Northeast Croatia, were screened for tolerance to PVY and TMV. Comparative tests were designed as randomized block with 4 repetitions, twenty plants in each. In the conditions of natural infection the number of plants with characteristic symptoms of PVY and TMV was registrated. The possible number of plants with characteristic symptoms was between 0 and 80. The cultivars used as a standard in all comparative tests were Podravina and Drava. They had the lowest number of plants with PVY symptoms. A similar number was found in the genotypes Virginia D (France), NC 744 (USA), and LP 4573 (Tobacco Inst. Zagreb). In the cultivars Delgold, NC 2326, McNair 944, McNair 373, NC 567, Coker 8 6, Kutsaga 110, there were many plants with PVY symptoms. There were more plants with PVY symptoms in the populations of F1 hybrids : Delgold x NC 744, K 394 x NC 744, McNair 944 x NC 744. The number of plants with TMV symptoms in the Podravina and Drava cultivars, in 1987, was between 5 and 30, in 1988 between 20 and 60. Varieties NC 567, Coker 86, Kutsaga 110 are described as resistant to TMV. There were no plants with TMV symptoms in the NC 567 and Coker 86 populations, both years. In the Kutsaga 110 cultivar, 1987, there were no plants with TMV symptoms either, but in 1988 there were six. The number of plants with characteristic TMV symptoms in other genotypes was similar to the number registrated in the Podravina and Drava cultivars.