For several years the I.T.T. in Sevilla has been working on obtaining new lines of tobacco, low in nicotine content, by the induced androgenesis procedure. The techniques used, with some variants, are those which J. P. Bourgin and J. P. Nitsch described in 1967. For this procedure we have used a great number of haploid plants of Nicotiana tabacum L., which originated from unripened grains of pollen which we have doubled in a solution of 0.2% of colchicine in methylcellulose. The chromosome evaluation was done in radical meristems by means of staining with orcine hydrochloride. The Nicotiana tabacum 2n plants which were utilized as pollen bearers belonged to the Virginia Bright K-4 variety and the hybrids in F3 which we grew during the years 1966 and 1969. The haploid plants and the diploid plants, with low alkaloid contents that we utilized for this procedure were found during the time we selected and adopted our cultivation.