A special study was conducted with the aim of evaluating the effects of pesticide treatment on tobacco in comparison with tobacco not treated with any pesticide. For the purpose of growing these tobaccos, experimental plots were selected on Prince Edward Island, Canada, where contamination of air, soil and water was at a minimum. The tobacco leaf was analyzed for 35 components and 28 pesticide residues. These samples are to be used for smoke analysis and bioassay. The results will be reported in a later publication.