Tobacco planting is especially responsive to the environment mainly due to the impacts of soil fluctuations on quality and quantity. The availability of environment realisations with an adequate accuracy is an essential component of tobacco production. In this paper, physical characteristics and the major chemical components of C3F flue-cured tobacco from five counties at a relatively similar latitude in the southern Yunnan Gold Corridor Tobacco Cultivation Region from 2013 to 2021 were analysed using Canonical Correspondence Analysis. The result shows the significant differences in the contents of organic matter, total nitrogen, total phosphorus, total potassium, and hydrolysed nitrogen as well as pH value of the soils; also in the thickness, stem ratio, leaf density, equilibrium moisture content, filling power and contents of phosphorus of the tobacco leaves from five areas. The findings underscore the importance of environment effects on physical characteristics. The total phosphorus content and pH value were obviously positive while available phosphorus, total nitrogen and total potassium content had a negative effect on the physical characteristics; available phosphorus, hydrolysed nitrogen, total phosphorus, total potassium, total nitrogen and pH value were the soil factors that most contributed to the tobacco chemical characteristics. It would be of benefit for the utilisation of flue-cured tobacco resources effectively with characteristics for different style cigarette products. On the other hand, suggestion of cultivation techniques would be supported for high-quality flue-cured tobacco.