Tobacco Science & Technology, 2011, 11, p. 71-75., ISSN.1002-0861
Extraction of genome DNA and optimization of RAPD reaction system of Rhizoctonia solani
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R. China
The genome DNAs of 16 strains of Rhizoctonia solani isolated from tobacco target spot were extracted by the improved CTAB method. The OD260/OD280 values of the obtained DNAs ranged from 1.76 to 1.89, the electrophoretic bands were clear and free of...