CORESTA Meeting, Agronomy/Phytopathology, Suzhou, 1999, AP51
Rapid creation of a new cytoplasmic male sterile line of flue-cured tobacco by interspecific cybridization
Qingzhou Tobacco Research Institute of CNTC, Qingzhou, Shandong, China
Mesophyll protoplasts isolated from N. tabacum var. K326 and inactivated by Rhodamine-6G (5 µg/ml, 15 minutes) were induced by PEG (6000) to fuse with those from N. repanda (without any treatment) under high Ca2+, high pH conditions. All 31...