Tob. Sci., 1991, 35-14, p. 59-62, ISSN. 0082-4523
Fractionation of flue-cured tobacco samples using Sep-Pak cartridges
Chemistry Laboratory, Agriculture Canada, Research Station, Delhi, Ontario, Canada
A method is described for the extraction and separation of tobacco constituents with C18 Sep-Pak™ cartridges. The first fraction was eluted with 60% aqueous methanol and contained most of the polar materials such as phenolic constituents, soluble...