The workshop combined six presentations illustrating different approaches and aspects o the assessment of ENDS ingredients with a 30-minute panel discussion where presenters will answer questions/discuss topics from the workshop participants. The six presentations not only described traditional toxicological approaches to ENDS ingredient evaluation (e.g., toxicological literature review, ingredient thermal degradation analysis, in vitro, in vivo testing) including advanced read across techniques, but several also encompassed toxicology relevant new assessment methods. These new assessment methods include in vitro assays for assessment of respiratory sensitization, use of more realistic 3D tissue constructs and aerosol delivery systems.

• Irene Abraham (JT International) - A tiered approach to ENDS ingredient assessment
• Florence Vonmoos & Karsta Luettich (Philip Morris) - Establishing an ENDS in vitro and in vivo ingredient assessment strategy using a toolbox approach
• Liam Simms (Imperial Brands) - A product stewardship assessment strategy for e-liquids
• Arno Gutleb (Invitrolize) - New assessment methods for ENDS ingredients
• Richard Savory (BAT Investments) - Evaluation of synthetic cooling agends WS-3 and WS-23 in 90-day inalation toxicity studies in Sprague Dawley rats
• Robert Leverette (RAI Services Company) - Toxicological assessment of electronic nicotine delivery systems using several 2D and 3D in vitro cytotoxicity and genotoxicity assays