A dwarf mutant with abundant suckers was observed in populations of the second generation of Nicotiana tabacum (L) cv. GT11, which experienced space mutation in a recoverable satellite, the Chinese third unmanned spacecraft "Shen-Zhuo III", and expressed readily in the field, then named as T-srd (Tobacco of Sucker-Rich and Dwarf mutant). The suckers of T-srd emerged at the first leaf node 5 weeks after sowing. In the field, the emergence of suckers was orderly from bottom to top, and in the grasp of the apical bud, which had no sucker until the node lay under half of the stalk height at first flower stage. At 90 days after transplanting, the plant height, length of internode, stem girth in T-srd were lower than those in GT11. There was no sucker-rich and dwarf phenotype in F1 population, and the segregation ratio in F2 population of seedling between sucker-rich and sucker-free phenotype corresponded with Mendel's Law based on the Chi-square test. Those results suggest that the sucker-rich and dwarf trait is a qualitative character that belongs to the recessive mutation of a single gene. Amplified fragment length polymorphism (AFLP) analysis showed that the difference between T-srd and GT11 existed on a molecular level. This mutant provides a useful material for research into sucker regulation in tobacco.