Tobacco Science & Technology, 2011, 10, p. 65-69., ISSN.1002-0861
Development and preliminary application of one-step RT-PCR kit for detection of tobacco CMV
1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, P.R. China
To detect tobacco CMV quickly and accurately, a pair of specific primers was designed according to the relatively conservative region in the CMV genomic sequence, and enzymes and buffers needed for reverse transcription and PCR were integrated. By ...