Search Results

11 results

  1. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 05

    Cloning and gene structure analysis of T-psy1 from flue-cured tobacco

    CAI Liuti(1); HU Zhongyi(1); LEI Lu(1); LI Jixin(2)
    (1) Guizhou Tobacco Research Institute, Guizhou Provincial Tobacco Company, Guiyang, P.R China; (2) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Carotenoid is an important precursor of tobacco aroma, and phytoene synthase is one of the key enzymes in the pathway of aroma precursors biosynthesis and accumulation in tobacco. Based on the homology cloning method, the cDNA and DNA sequence of the ...
  2. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light aroma ...
  3. Tobacco Science & Technology, 2011, 1, p. 60-65., ISSN.1002-0861

    Comparison of major components in leaf trichome exudate of flue-cured tobacco grown under different climatic conditions

    ZHU XIAN-LING(1); PAN WEN-JIE(2); CHEN YI(2); CHEN WEI(2); LI ZHANG-HAI(1); ZHANG JI-LI(1); XU ZENG-HAN(1); LI HONG-XUN(2)
    1. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, P.R. China; 2. Guizhou Tobacco Science Research Institute, Guiyang 550023, P.R. China
    In order to find out the effects of climatic factors on leaf trichome exudates, a field experiment on flue-cured tobacco cv. Yun 85 was conducted at Longgang of Kaiyang County, Weining County and Tianzhu County in Guizhou Province. Ripe leaves harvested ...
  4. Tobacco Science & Technology, 2011, 12, p. 26-33., ISSN.1002-0861

    Calculation of ionization energy of cigarette smoke component and its applications in qualitative analysis

    WANG CHENG-HUI(1); HU YONG-HUA(1); WANG JIAN(2); XU YING-BO(1); PAN YANG(2)
    1. Technology Center, China Tobacco Anhui Industrial Co., Ltd., Hefei 230088, P.R. China; 2. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, P.R. China
    : In order to systemically evaluate the reliability of the method of predicting ionization energy with density functional method in quantum chemistry [B3LYP/6-311++G(d,p)] and composite ab initio (G3B3), 70 chemical portions of known ionization energy in ...
  5. Tobacco Science & Technology, 2011, 12, p. 52-55., ISSN.1002-0861

    Simultaneous determination of five neonicotinoids pesticide residues in tobacco leaf with SPE-LC/MS/MS

    HE QING; XU YING-BO; WANG CHENG-HUI; TIAN ZHEN-FENG; ZHU DONG-LIANG
    Technology Center, China Tobacco Anhui Industrial Corporation, Hefei 230088, P.R. China
    By comparing the affinities of silica gel, graphitized carbon, C 18, and polymer cartridges for the analyzed pesticides, a method for simultaneously determining five neonicotinoid pesticides, including nitenpyram, thiamethoxam, imidacloprid, acetamiprid ...
  6. Tobacco Science & Technology, 2011, 12, p. 61-64., ISSN.1002-0861

    Reaction of tobacco to oxidation stress induced by topping and bionic signal molecules

    ZHANG XIN-HUA(1,2); YAO ZHONG-DA(3); CHEN CHANG-WEI(1); CHA XIANG-DONG(2); CHENG XIN-SHENG(1)
    1. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China; 2. Biological Science College of Anhui University, Hefei 230039, P.R. China; 3. China Tobacco Anhui Industrial Co., Ltd., Hefei 230088, P.R. China
    To investigate whether the mechanical injury on tobacco by topping induces oxidation stress, the contents of superoxide anion (O 2- •), hydrogen peroxide (H 2 O 2) and malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in leaf tissue ...
  7. Tobacco Science & Technology, 2011, 2, p. 35-38., ISSN.1002-0861

    Comparison of combustion behavior and gas phase pyrolytic products between flue-cured, Burley and Oriental tobacco

    ZHOU SHUN; XU YING-BO; WANG CHENG-HUI; SHU JUN-SHENG; SHE SHI-KE; TIAN ZHEN-FENG
    Technology Centre, China Tobacco Anhui Industrial Corporation, Hefei 230088, P.R. China
    Micro-scale combustion calorimetry (MCC) and thermogravimetric analysis coupled with Fourier transform infrared spectrometer (TG-FTIR) methods were applied to investigate the combustion behavior of flue-cured, burley and oriental tobaccos, as well as the ...
  8. Tobacco Science & Technology, 2011, 2, p. 58-63., ISSN.1002-0861

    Cloning and sequence analysis of T-psy1 gene from flue-cured tobacco

    CAI LIU-TI(1); JIANG GUANG-HUA(2); LEI BO(1); HU ZHONG-YI(1)
    1 .Guizhou Tobacco Research Institute, Guiyang 550018, P.R. China; 2. Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    Adopting a homology cloning method, the full length cDNA sequence of tobacco phytoene synthase gene (named T-psy1) was cloned from flue-cured tobacco cv. K326, the T-psy1 gene GenBank accession number HM345582, included 1521bp, with coding protein ...
  9. Tobacco Science & Technology, 2011, 9, p. 37-40., ISSN.1002-0861

    Influencing factors and improvement of determination of hydrogen cyanide in mainstream cigarette smoke by autoanalyzer

    QU XIAN-ZHONG; CHEN KAI-BO; XU ZHI-QIANG; WANG CHENG-HUI
    Technology Center, China Tobacco Anhui Industrial Corporation, Hefei 230088, P.R. China
    In order to determine hydrogen cyanide (HCN) in mainstream cigarette smoke with autoanalyzer more accurately, the effects of formaldehyde, acetaldehyde, acetone and acrolein, as well as the concentration of formaldehyde on the determination system were ...