1. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  2. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 12

    Biocontrol efficiencies and identification of nematicidal metabolite from strain BJLSH9 towards nematode and Phytophthora nicotianae

    DUAN Yan-qing(1); LI Ping(2); ZHE Wei(1); XI Jia-qin(3); XIA Jianjun(1); WANG Wenyuan(1); JIANG Junxiang(1); MO Ming-he(2)
    (1) Technology Center of Hongyun Honghe Tobacco (Group) Co., Ltd, Wuhua District, Kunming, P.R. China; (2) Key Laboratory for Conservation and Utilisation of Bio-Resources, Yunnan University, Kunming, P.R. China; (3) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, P.R. China
    BJLSH9, an actinomycete exhibiting both nematicidal and antifungal activities, was isolated from rhizospheric soil of willow in Beijing. Bioassay results in vivo showed that BJLSH9 displayed a 50.05% efficiency to suppress radial growth of Phytophthora ...
  3. CORESTA Congress, Sapporo, 2012, Plenary Session, abstr. IG 02

    Levels of tobacco-specific N-nitrosamines NNN and NNK in commercial cigarettes sampled in2008-2010 – A review of analytical data

    GUNDUZ I.(1); KONDYLIS A.(1); HOFER R.(1); JACCARD G.(2); RUFFIEUX L.(3); GADANI F.(1)
    (1) Philip Morris Products SA, Product Development – Applied Cigarette Research, Neuchatel, Switzerland; (2) Philip Morris International Management SA, Product and Process Quality, Neuchatel, Switzerland; (3) Philip Morris International Management SA, Agricultural Programs, Lausanne, Switzerland
    The present study was designed to determine levels of the tobacco-specific N-nitrosamines (TSNAs) N’-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in tobacco cut filler and in mainstream smoke of commercial cigarette ...
  4. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, PT 05

    Impact of cigarette paper properties on smoke constituents’ delivery under Health Canada Intense smoking regime

    GUYARD A.; MEIER D.; CECCHETTO A.; HOFER R.; LI P.
    Philip Morris Products SA, Product Development – Applied Cigarette Research, Neuchatel, Switzerland
    This investigation was undertaken to assess to which extent the changes of cigarette paper characteristics may impact the delivery of nine cigarette smoke constituents when measured under Health Canada Intense (HCI) smoking regime and normalised to smoke ...
  5. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, PT 07

    Improved methods for the determination of crop protection agent residues in tobaccos and tobacco products by LC-MS/MS using the QuEChERS extraction

    DEGER A.; JACCARD G.
    Philip Morris International Management SA, Product and Process Quality, Neuchatel, Switzerland
    We will present two improved methods to determine Crop Protection Agent (CPA) residues in tobaccos and tobacco products and discuss their development and validation. The methods are applicable to 53 of the 118 CPA recommended in the CORESTA Guide No. 1 [1 ...
  6. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SS 02

    Development of a systems toxicology approach and its application to quantify the biological impact of tobacco smoke in vitro and in vivo

    PEITSCH M.C.; MATHIS C.; WAGNER S.; KOGEL U.; POUSSIN C.; SCHLAGE W.; STINN W.; MEURRENS K.; WEISENSEE D.; GEBEL S.; BELCASTRO V.; XIANG Y.; MARTIN F.; SEWER A.; HENGSTERMANN A.; ANSARI A.; HOENG J.
    Philip Morris International R&D, Philip Morris Products SA, Neuchâtel, Switzerland
    The potential health risks of long-term exposure to biologically active substances such as therapeutic drugs or environmental toxins are frequently determined a posteriori through clinical epidemiology studies. However, disease may take decades to ...
  7. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 28

    CSEO – The cigarette smoke exposure ontology

    YOUNESI E.(1), COGGINS C.(2); ANSARI S.(3); HOENG J.(3); PEITSCH M.(3); HOFMANN-APITIUS M.(1)
    (1) Fraunhofer Institute for Algorithms and Scientific Computing SCAI, Sankt Augustin, Germany; (2) Carson Watts Consulting, King, NC, USA; (3) Philip Morris International R&D, Neuchâtel, Switzerland
    In the past years, significant progress has been made in the development and use of experimental settings for collection of massive and precise experimental data on tobacco exposure and the diseases induced by it. Despite the growing number of such data, ...
  8. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain ...
  9. Tobacco Science & Technology, 2012, 4, p. 56-59, 66, ISSN.1002-0861

    Analysis of residues of famoxadone and fenamidone in tobacco

    ZHU Baokun(1); LIAO Yuqi(2); JIANG Juxing(1); WANG Sujuan(1); LIU Ya(1); XIA Jianjun(*1)
    (1) Hongyun-Honghe Tobacco (Group) Co., Ltd., Kunming 650202, P.R. China; (2) School of Chemical Science and Technology, Yunnan University, Kunming 650091, P.R. China
    A method for fast testing the famoxadone and fenamidone residues in tobacco leaves with ultra-high performance liquid chromatography was developed by using acetonitrile as an extraction solvent. Twenty-two flue-cured tobacco samples were tested. The ...
  10. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...