Search Results

15 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, ST 64

    A novel approach for the screening of e-cigarette aerosols using an Ames whole aerosol assay

    THORNE D.(1); HOLLINGS M.(2); SEYMOUR A.(2); CROOKS I.(1); MEREDITH C.(1); GAҪA M.(1)
    (1) British American Tobacco (Investments) Limited, R&D, Southampton, U.K.; (2) Covance Laboratories Ltd, Harrogate, North Yorkshire, U.K.
    The in vitro mutagenic potential of the aerosol from Vype ® ePen e-cigarette was assessed using the Ames assay. E-cigarette aerosol was trapped on a Cambridge filter pad, eluted in DMSO and compared to cigarette smoke total particulate matter (TPM), ...
  2. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 34

    The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

    THORNE D.(1); CROOKS I.(1); HOLLINGS M.(2); SEYMOUR A.(2); MEREDITH C.(1); GAÇA M.D.(1)
    (1) British American Tobacco, R&D Centre, Southampton, U.K.; (2) Covance Laboratories Ltd, Harrogate, U.K.
    Salmonella typhimurium strains TA98 and TA100 were used to assess the mutagenic potential of a commercially available rechargeable, dual voltage, closed system modular electronic-cigarette (Vype ® ePen, Nicoventures UK). Results were compared to a ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, STPOST 28

    The comparative assessment of cigarette smoke with an in vitro BALB/c cytotoxicity test using altered vapour phase delivery products

    THORNE D.; DILLON D.; DALRYMPLE A.; DUKE M.; MEREDITH C.
    British American Tobacco, R&D Centre, Southampton, U.K.
    The objective of this study was to evaluate a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. The functionality and applicability of this modified protocol was assessed by comparing the ...
  4. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, STPOST 20

    The assessment of the mutagenic potential of 3R4F mainstream cigarette smoke using multiple Ames strains

    THORNE D.(1); KILFORD J.(2); HOLLINGS M.(2); DALRYMPLE A.(1); BALLANTYNE M.(2); MEREDITH C.(1); DILLON D.(1)
    (1) British American Tobacco, Group R&D, Southampton, U.K.; (2) Covance Laboratories Ltd, Harrogate, U.K.
    The Ames methodology is governed by clear international regulatory guidelines (e.g. OECD 471), which recommend the use of at least five bacterial strains in an in vitro test battery. Salmonella typhimurium strains TA1535; TA1537 or TA97 or TA97a; TA98 and ...
  5. TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 01

    Integrating chemical, toxicological and clinical research to assess the potential of reducing health risks associated with cigarette smoking through toxicant regulation

    PROCTOR C.; SHEPPERD C.; McADAM K.; ELDRIDGE A.; MEREDITH C.
    British American Tobacco, Group Research and Development Centre, Regents Park Road, Southampton, UK
    The concept of a risk continuum for tobacco and nicotine products has been proposed that represents the likely association of different categories of product with toxicant exposure and possibly risk. At one end are cigarettes, deemed the most risky, and ...
  6. CORESTA Meeting, Smoke Science/Product Technology, 2013, Seville, STPOST 15

    A modified Ames methodology for the assessment of mainstream cigarette smoke genotoxicity using an aerosol-based exposure system

    THORNE D.(1); KILFORD J.(2); PAYNE R.(2); DALRYMPLE A.(1); CLEMENTS J.(2); MEREDITH C.(1); DILLON D.(1)
    (1) British American Tobacco, Group R&D, Southampton, Hampshire, U.K.; (2) Covance Laboratories Ltd, Harrogate, North Yorkshire, U.K.
    To date most toxicological testing of cigarettes has been performed on the particulate phase of cigarette smoke using standard genotoxic and cytotoxic methods, which include the AMES reverse mutation assay, neutral red uptake, mouse lymphoma and ...
  7. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 13

    Further refinement of a margin of exposure (moe) model to prioritize cigarette smoke toxicants for reduction research.

    BREHENY D.; CUNNINGHAM F.H.; FIEBELKORN S.; DILLON D.; MEREDITH C.; PROCTOR C.
    British American Tobacco Ltd., Southampton, UK
    The US Food and Drug Administration (FDA) have outlined seven research priority areas relating to tobacco products. Research area three focused on “reducing toxicity and carcinogenicity of tobacco products and smoke”. This has been coupled with an ...
  8. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 02

    Tools for the prioritisation of tobacco smoke toxicants: an overview.

    CUNNINGHAM F.; FIEBELKORN S.; DILLON D.; MEREDITH C.
    British American Tobacco, Group Research & Development, Southampton, UK
    A scientific, evidence-based risk assessment method to assess potential health risks of individual tobacco smoke toxicants, reflecting the range of yields and human characteristics related to exposure, is a beneficial tool for tobacco product regulation ...
  9. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 10

    Dosimetric assessment of whole smoke particulate deposition in vitro: a proposed common approach using quartz crystal microbalance technology.

    ADAMSON J.; THORNE D.; DALRYMPLE A.; MEREDITH C.; DILLON D.
    British American Tobacco, Southampton, UK
    There are a number of different smoking machines and exposure chamber combinations used by our industry to assess the toxicological impact of cigarette smoke in vitro. The amount of smoke delivered to cells within an in vitro exposure system can be ...
  10. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 20

    An in vitro comparative study of [14c]-eugenol and [14c]-methyleugenol activation and detoxification kinetics in human, mouse, and rat liver fractions.

    MINET E.(1); GENTILE D.(2); MEREDITH C.(1); MASSEY E.D.(1)
    (1) British American Tobacco, Group R&D, Southampton, UK; (2) Charles River Laboratories, Edinburgh, UK
    Introduction- Eugenol is a natural alkenylbenzene compound used in a variety of consumer products including Kretek cigarettes. There is limited evidence for the carcinogenicity of eugenol to experimental animals. However, in vitro tests for the genotoxic ...