1. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, AP 05

    Characterisation of leaf tobacco with reduced amount of sugar by RNAi-mediated gene silencing of ADP-glucose pyrophosphorylase small subunit

    SUZUKI S.(1); SAITO Y.(1); FUJISAKI T.(2); HIYOSHI T.(1)
    (1) Japan Tobacco Inc., Leaf Tobacco Research Center, Oyama, Tochigi, Japan; (2) Japan Tobacco Inc., R&D Planning Div., Tokyo, Japan
    Reportedly, good correlation exists between sugars in cigarettes and carbonyls in mainstream smoke. We reported that sugar and starch contents of flue-cured tobacco leaves were decreased by reduced expression of genes related to starch biosynthesis under ...
  2. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 28

    Decrease in the carbohydrate (starch and sugar) content of flue-cured tobacco leaves by reduced expression of the genes related to starch biosynthesis

    SUZUKI S.(1); NOGUCHI S.(1); YAMAGUCHI N.(1); FUJISAKI T.(2); HIYOSHI T.(1)
    (1) Japan Tobacco Inc., Leaf Tobacco Research Center, Oyama, Tochigi, Japan; (2) Japan Tobacco Inc., R&D Planning Div., Tokyo, Japan
    Starch and sugar (carbohydrate) contents of flue-cured tobacco leaves produced in Japan, which are higher than in other countries, are related to the low filling capacity of cut rag. Reducing the contents is expected to improve its applicability to ...
  3. CORESTA Congress, Shanghai, 2008, AP 14

    EST analysis of tobacco normalized full-length cDNA library and preparation of tobacco microarray

    SUZUKI S.; MATSUURA D.; NOGUCHI S.; HIYOSHI T.; NAKAKIDO F.
    Japan Tobacco Inc., Leaf Tobacco Research Lab., Oyama, Tochigi, Japan.
    Full-length cDNAs are useful for the accurate annotation of gene structures in genomic DNA and use in functional assays. We prepared a Nicotiana tabacum normalized full-length cDNA library and sequenced both 5' and 3' ends of cDNAs. The library ...