1. CORESTA Meeting, Smoke Science/Product Technology, Jeju, 2007, SSPT 43

    Detection of cytotoxicity in the water-insoluble fraction of cigarette smoke gas vapor phase using a whole smoke exposure system

    NISHINO T.; NARA H.; FUKANO Y.
    Japan Tobacco Inc., Tobacco Science Research Lab., Yokohama, Kanagawa, Japan.
    When biological evaluations of cigarette smoke are conducted, the particulate phase (PP) and gas vapor phase (GVP) of mainstream smoke are collected separately and exposed to cultured cells. GVP is generally collected in phosphate buffer saline (PBS) by ...
  2. CORESTA Congress, Paris, 2006, SS 31

    Differences in the generation of 8-hydroxyguanine by cigarette smoke in calf-thymus DNA and in A549 cells

    MIURA N.; FUKANO Y.; NISHINO T.
    Japan Tobacco Inc., Tobacco Science Research Lab., Yokohama, Kanagawa, Japan.
    In this report we investigated the generation of 8-hydroxyguanine by cigarette smoke using calf-thymus DNA and A549 cells. At first, we exposed the calf-thymus DNA to cigarette smoke bubbled PBS and measured the amount of 8-OhdG, which is the hydrolysate ...