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  1. Bull. Spec. CORESTA Congress, Vienna, 1984, p. 95, A19, ISSN.0525-6240

    Cytoplasmic substitution by means of protoplast fusion _ a quick method of obtaining cytoplasmic male sterility in tobacco

    KUMASHIRO T.; KUBO T.
    Japan Tobacco and Salt Public Corp. Iwata Tobacco Experiment Station, Iwata, Shizuoka-ken, Japan
    Protoplasts of Nicotiana debneyi were inactivated by X-ray irradiation (10 KR) and were fused with a recessive chlorophyll mutant (consolation 402) tobacco (N. tabacum) protoplasts. Under no selection pressure on the PEG-treated protoplasts, two types of ...