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  1. Bull. Spec. CORESTA Symposium, Kallithea, 1990, p. 144, A35, ISSN.0525-6240

    Morphocytological characteristics of a somatic hybrid of Nicotiana rustica X Nicotiana tabacum

    SORRENTINO C.; LOMBARDO G.; DI MURO A.
    Istituto Sperimentale per il Tabacco, Scafati, Italy
    Protoplasts from foliar mesophyll of N. rustica and N. tabacum seedlings, aseptically cultured, were treated with a 50% PEG 6000 solution for ten minutes at room temperature, diluted with the culture medium and gently shaken. They were then cultured in ...