Bull. Spec. CORESTA Symposium, Kallithea, 1990, p. 144, A35, ISSN.0525-6240
Morphocytological characteristics of a somatic hybrid of Nicotiana rustica X Nicotiana tabacum
Istituto Sperimentale per il Tabacco, Scafati, Italy
Protoplasts from foliar mesophyll of N. rustica and N. tabacum seedlings, aseptically cultured, were treated with a 50% PEG 6000 solution for ten minutes at room temperature, diluted with the culture medium and gently shaken. They were then cultured in ...