Search Results

13 results

  1. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 049

    Assessment of in vitro toxicity/genotoxicity of ends and combustible products

    JORDAN K.(1); BOMBICK B.(1); COOPER B.(1); LEVERETTE R.(1); SHUTSKY T.(1); WEST C.(1); STRASSER J.(1); ROUND E.(1); MARANO K.M.(1); MOMIN R.(2); TRIVEDI A.(2); WAGSTAFF W.(2); BRUCE S.(3); DAKOULAS E.(3); ROY S.(3)
    (1) RAI Services Company, Winston-Salem, NC, USA; (2) Labstat International ULC, Kitchener, ON, Canada; (3) BioReliance Corp, Rockville, MD, USA
    In May 2016, the U.S. Food and Drug Administration Center for Tobacco Products (CTP) issued draft guidance for Premarket Tobacco Product Applications (PMTA) for Electronic Nicotine Delivery Systems (ENDS), which recommends inclusion of in vitro...
  2. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 32

    Cellular transformation activity of different tobacco condensates in the Bhas 42 cell assay

    STEVENSON M.(1); WIECZOREK R.(2); SIMMS L.(1); PANT K.(3)
    (1) Imperial Tobacco Limited, Bristol, U.K.; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) BioReliance, Sigma-Aldrich Corp., Rockville, MD, U.S.A.
    Carcinogenesis has been described as a multi-stage process comprising of initiation, promotion and progression. Smoking is a cause of serious disease in smokers, including lung cancer. Determining the carcinogenic potential of a tobacco product...
  3. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 35

    Comparison of the carcinogenic potential of tobacco smoke and electronic-cigarette aerosol using the Bhas cell transformation assay

    BREHENY D.(1); OKE O.(1); PANT K.(2); BRUCE S.W.(2); LAFORCE M.K.(2); CELESTIN N.(2); GAÇA M.D.(1)
    (1) British American Tobacco, R&D Centre, Southampton, U.K.; (2) BioReliance, Sigma-Aldrich Corp., Rockville, MD, U.S.A.
    In vitro cell transformation assays (CTA) are used to assess the carcinogenic potential of chemicals and complex mixtures. They can detect non-genotoxic as well as genotoxic carcinogens. The Bhas 42 CTA has been developed with both initiation and...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2011, Graz, STPOST 06

    Evaluation of cigarette smoke-induced cell transformation using three in vitro cell transformation assays

    FOWLER K.; FIELDS W.R.; NORDSKOG B.K.; POTTS R.; BOMBICK B.R.; PANT K.
    (1) R.J. Reynolds Tobacco Co., R&D, Winston-Salem, NC, USA; (2) BioReliance Corporation, Rockville, MD, USA
    Cell transformation assays (CTA) detect phenotypic changes associated with neoplastic transformation and available evidence suggests in vitro cell transformation may stimulate early components of the multistage process of carcinogenesis. Moreover,...
  5. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 22

    Cell transformation activity of cigarette smoke condensate in Bhas 42 ASSAY.

    PANT K.(1); BRUCE S.W.(1); GAIROLA C.G.(2)
    (1) BioReliance Corp Rockville, MD, USA; (2) University of Kentucky Medical Center, Lexington, KY, USA
    Cell transformation assays have been commonly used to predict chemical carcinogenicity. Using v-Ha-ras transfected BALB/c3T3 (Bhas 42) cells, Sasaki and coworkers (2010, 2011) have recently validated a sensitive short-term assay that simulates the...
  6. Bull. Spec. CORESTA Congress, Lisbon, 2000, p. 107, APOST6 (adjourned)

    Estimation of the state of nitrogen nourishment of tobacco using the N-tester

    KOWALCZYK-JUSKO A.; KOSCIK B.
    University of Agriculture, Lublin - Institute of Agricultural Sciences, Zamosc, Poland.
    The purpose of this work, based on a three years of field experiment, was to inspect whether it is possible to use a chlorophyll meter in fixing the nitrogen doses used in growing flue-cured tobacco. The relationship between the capacity of nitrogen...
  7. Bull. Spec. CORESTA Congress, Brighton, 1998, p. 112, APOST13

    Influence of aqua terra hydrogel on growth, development and yield of light cigarette tobacco

    KOSCIK B.; KOWALCZYK-JUSKO A.
    University of Agriculture, Lublin - Institute of Agricultural Sciences, Zamosc, Poland.
    The aim of introductory field and pot experiments which were carried out in Zamosc in the years of 1995-1997 was to study the influence of Aqua Terra hydrogel on the growth, development and yield of light cigarette tobacco. Hydrogels are substances...