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221 results

  1. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SS 02

    Development of a systems toxicology approach and its application to quantify the biological impact of tobacco smoke in vitro and in vivo

    PEITSCH M.C.; MATHIS C.; WAGNER S.; KOGEL U.; POUSSIN C.; SCHLAGE W.; STINN W.; MEURRENS K.; WEISENSEE D.; GEBEL S.; BELCASTRO V.; XIANG Y.; MARTIN F.; SEWER A.; HENGSTERMANN A.; ANSARI A.; HOENG J.
    Philip Morris International R&D, Philip Morris Products SA, Neuchâtel, Switzerland
    The potential health risks of long-term exposure to biologically active substances such as therapeutic drugs or environmental toxins are frequently determined a posteriori through clinical epidemiology studies. However, disease may take decades to ...
  2. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 28

    CSEO – The cigarette smoke exposure ontology

    YOUNESI E.(1), COGGINS C.(2); ANSARI S.(3); HOENG J.(3); PEITSCH M.(3); HOFMANN-APITIUS M.(1)
    (1) Fraunhofer Institute for Algorithms and Scientific Computing SCAI, Sankt Augustin, Germany; (2) Carson Watts Consulting, King, NC, USA; (3) Philip Morris International R&D, Neuchâtel, Switzerland
    In the past years, significant progress has been made in the development and use of experimental settings for collection of massive and precise experimental data on tobacco exposure and the diseases induced by it. Despite the growing number of such data, ...
  3. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain ...
  4. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  5. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2 kD ...
  6. Tobacco Science & Technology, 2012, 7, p. 72-75, ISSN.1002-0861

    Analysis of differential protein in tobacco leaves sprayed with microbial agent MP

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2)
    (1) Technology Center, China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China
    In order to find out the effects of Microbial agent MP (Bacillus magaterium) spraying on the quality of tobacco leaves, two-dimensional gel electrophoresis (2-DE) was used to analyze the differences of protein expression between tobacco leaves sprayed ...
  7. Tobacco Science & Technology, 2012, 9, p. 58-62, ISSN.1002-0861

    Selective removal of hydrogen cyanide from cigarette smoke by combined filter of coptis alkaloid

    WU Jinfeng(1,2); LI Xuegang(1); WANG Peng(3); PENG Zhong(3); LAN Zhongyu(3); ZHENG Lifeng(2)
    (1) College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P.R. China; (2) Chongqing Cigarette Factory, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400061, P.R. China; (3) Department of Technology, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400060, P.R. China
    Coptis alkaloids were extracted from rhizome of Coptis chinensis rich in quaternary ammonium hydroxide and added into cigarette filter. On the basis of the reduction of hydrogen cyanide delivery in cigarette smoke, the way of coptis alkaloid adding into ...
  8. Tobacco Science & Technology, 2012, 9, p. 75-81, ISSN.1002-0861

    Cloning and sequence analysis of carotenoid cleavage dioxygenase gene from Nicotiana tabacum

    GAO Junping(1); WANG Genhong(1,2); CHENG Tingcai(1,2); DUAN Jun(1,2); XIA Qingyou(*2)
    (1) Institute of Agriculture and Life Science, Chongqing University, Chongqing 400044, P.R. China; (2) State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, P.R. China
    In order to reveal the function of carotenoid cleavage dioxygenases (CCDs) in the growth and development of tobacco plants, a CCD4 (NtCCD4) gene was cloned from Samsun oriental tobacco by RT-PCR. According to sequence analysis, with a coding sequence of ...
  9. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light aroma ...
  10. CORESTA Meeting, Smoke Science/Product Technology, 2011, Graz, STPOST 07

    Evaluation of the diagnostic accuracy of a candidate biomarker to detect use of conventional cigarettes in users of tobacco products that do not burn tobacco

    MARTIN LEROY C.(1); LINDNER D.(2); HAZIZA C.(2); FORTE N.(2); MAGNETTE J.(2)
    (1) Clinopsis SA, Concise, Switzerland; (2) Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland
    Objective: In clinical studies to assess alternative tobacco products that heat rather than burn tobacco, a compliance biomarker is needed to detect the additional use of conventional cigarettes. Standard biomarkers of exposure to cigarette smoke ...