Recommended Methods current

No. 75 - Determination of Tobacco Specific Nitrosamines in Mainstream Cigarette Smoke by LC-MS/MS

(CRM developed into ISO 19290)

August 2019 - third edition Ref. SMA-232-CRM-75

(CRM developed into ISO 19290:2016)


This Recommended Method is applicable to the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-Nitrosonornicotine (NNN), N-Nitrosoanatabine (NAT), NNitrosoanabasine (NAB) and 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK).

The use of these machine smoking parameters reflects their inclusion in the reporting requirements of various national regulations rather than an endorsement of their appropriateness by CORESTA.


Related documents

CRM 63 - Determination of Tobacco Specific Nitrosamines in Cigarette Mainstream Smoke - GC-TEA Method (January 2019)

Report - Updates of CORESTA Recommended Methods after Further Collaborative Studies Carried Out under Both ISO and Health Canada Intense Smoking Regimes (December 2013)

Report - Determination of Tobacco Specific Nitrosamines in Cigarette Mainstream Smoke. The CORESTA 2011 Collaborative Study (December 2012)