Recommended Methods current

No. 75 - Determination of Tobacco-Specific Nitrosamines in Mainstream Smoke by LC-MS/MS

(CRM developed into ISO 19290; ISO 23921)

September 2022 - fourth edition Ref. SMA-SA-198-3-CRM-75

(ISO 19290 partially corresponds to this CRM)

This method is applicable to the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette and cigar smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-Nitrosonornicotine (NNN), N-Nitrosoanatabine (NAT), N-Nitrosoanabasine (NAB) and 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK).

The use of these machine smoking parameters reflects their inclusion in the reporting requirements of various national regulations rather than an endorsement of their appropriateness by CORESTA.

Related documents

CRM 102 - Determination of Tobacco-Specific Nitrosamines in E-Liquid by LC-MS/MS (January 2023)

CRM 65 - Determination of Total and Nicotine-Free Dry Particulate Matter using a Routine Analytical Cigar-Smoking Machine – Determination of Total Particulate Matter and Preparation for Water and Nicotine Measurements (August 2019)

CRM 64 - Routine Analytical Cigar-Smoking Machine - Specifications, Definitions and Standard Conditions (May 2018)

CRM 63 - Determination of Tobacco Specific Nitrosamines in Cigarette Mainstream Smoke - GC-TEA Method (January 2019)

CRM 46 - Atmosphere for Conditioning and Testing Cigars of all Sizes and Shapes (June 2018)

Report - 2019 Collaborative Study for B[a]P and TSNA Compounds in Mainstream Cigar Smoke (June 2022)

Report - Updates of CORESTA Recommended Methods after Further Collaborative Studies Carried Out under Both ISO and Health Canada Intense Smoking Regimes (December 2013)

Report - Determination of Tobacco Specific Nitrosamines in Cigarette Mainstream Smoke. The CORESTA 2011 Collaborative Study (December 2012)